Gene introgression in the non-tuber forming solanum
1999
Dela Vina, A.C. | Ramirez, D.A. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
Nineteen species of the non-tuber forming Solanum, including Solanum melongena, eggplant, obtained from the National Plant Genetic Resources Laboratory were used in the hybridization program with the aim of determining the genetic affinity among the species and ultimately transfer desirable genes (resistance to pests and diseases, prolificacy, tolerance to abiotic stresses) from the wild relatives to the cultivated eggplant. A total of 135 cross combinations were made from which only 43 cross combinations developed fruits with very few seeds recovered. Degree of genetic relationship was measured in terms of percentage seed developed and chromosomal behavior of the hybrids during meiosis. Percent seed recovery ranged from 0.0 percent to 80.0 percent. Meiotic analysis of the different species and hybrids gave a chromosome number 2n=24. The chromosome behavior of the parentals was generally normal. The hybrids on the other exhibited partial to complete homology among the chromosomes of the parental species. Laggards and bridges were observed in both parents and hybrids. Pollen fertility of the different species ranged from 77.37 to 98 percent whereas the hybrids have a range of 9.74 to 30.5 percent. Backcrossing to the melongena parent was done for four generations in cross aethiopicum x melongena. Partial morphological characterization of the hybrids showed gene introgression from S. aethiopicum to S. melongena
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