Morphology and electron microanalysis of inner organs in cattle kept in immission regions of east Slovakia
1999
Pivko, J. | Uhrin, V. | Galova,J. | Macak, V. | Pavlik, V. (Research Institute of Animal Production, Nitra (Slovak Republic))
The inner organs of cows (n=25) and bulls (n=10) kept in regions affected with emmissions were analysed histopathologically and electron microanalytically. The samples for light microscopy were fixed in 10% formol, dehydrated by ascendant series of alcohols and embedded into paraffin. The paraffin cuts were stained by Harrisn haematoxylin and 0.5% eosin. The preparations were evaluated through the light microscope Jenaval. For the electron microscopy were the samples fixed in 4% glutaraldehyde, in 1% osmium acid, washed in cacodylate buffer, dehydrated by ascendant series of alcohols and mounted into AGAR - 100. The ultrathin cuts were stained by lead citrate and uranyl acetate. The electrongrams were evaluated through the microscope JEM CX-100. For the analytical transmission microscopy were used 100 nm thick cuts, contrasted 5 minutes by uranyl acetate, dehydrated and steamed by thin layer of carbon. Analyses of 14 elements were performed through the electron microscope TEM 2000FX with EDS detector (LINK 10000/855) at 80 kV, electron flow 5 p A and time of extinction 50 seconds. We found out that the parenchymatic organs of cows living in polluted surroundings for long time are affected by various pathological processes. Nephroses, vacuolization of hepatocytes and interstitial pneumonia were the most common, however, they did not present themselves clinically. On the other hand, we did not observe such processes in the organs of young slaughter bulls. From the results the conclusion can be drawn that the contamination of the surroundings and feed is decisive and not the ways by which the harmful substances enter the organism. The results of quantitative analyses confirm that the cells of various organs contain different levels of the same elements
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par Agricultural Knowledge and Innovation Institute
Découvrez la collection de ce fournisseur de données dans AGRIS