Isolation and screening of lactic acid bacteria for enhancing silage fermentation efficiency
1997
Amnat Jarerat
Forty-nine lactic acid bacteria were isolated from natural sources, 40 isolates and 9 isolates, namely Pediococcus sp. and Lactobacillus sp. respectively. They were screened according to the characteristics for enhancing silage fermentation efficiency, using Lactobacillus plantarum IAM 1041 as a reference strain. The results clearly showed that Lactobacillus sp. I4 (TISTR 1024) isolated from laboratory silage sample was suitable for grass silage fermentation. It was homofermentative lactic acid bacteria, well growth in anaerobic condition with high lactic acid yield (90 percent) and low pH to 4.38 4.14 and 4.93 when glucose, fructose and sucrose were used as carbon sources. The 25 g of fresh grass was ensiled by using polypropylene plastic bag at 30 37 45 and 50 deg C resulting in maximum lactic acid production of 4.68 percent dry matter at 50 deg C for 1 day ensiling. Application of 10*[7) CFU of Lactobacillus sp. I4 to 1 g of fresh grass showed well preserved silage of pH 4.32 lactic acid 3.60 percent DM ammonia nitrogen 4.46 percent (total N2) and no butyric acid when silage fermentation was carried out at 45 deg C for 20 days. More over protein loss obtained from grass silage fermentation with inoculum was less than the natural fermentation for 4.8 times.
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