Using repetitive DNA sequence as a probe to detect genetic variation in rice blast fungus
1997
Poonsak Mekwatanakarn | Levy, M. | Zeigler, R.S. (Ubon Ratchathani Rice Research Center (Thailand))
Rice blast fungus (Pyricularia grisea Sacc.) were detected from trap nursery, grown in different cultivars base on upland short row technique. Single conidia were isolated from infected leave. Restriction fragment DNAs from each isolates were separated by electrophoresis. DNA were transferred to nylon membrane and hybridized with radioactive-labeled repetitive sequence probe (MGR586) and washed with high stringency and visualized by autoradiography. 40-60 DNA fingerprinting bands were scored visually. Phenogram of each isolates were created to compare similarity of the DNA bands by cluster analysis and boot trap analysis. 654 isolates collected from 5 trap nursery sites in the North, Northeast and Central region of Thailand in 1993-94 were typed for DNA fingerprinting. 51 lineages were detected among Thai rice blast fungal population. Lineage 2, 5 and 43 detected from 83, 37 and 50 isolates respectively were found in the North, Northeast and Central Thailand. The most common lineage found in lineage 2. The fungus in lineage 5 was the most diversity (diversity indix = 0.871). However diversity index in lineage 43 was 0.69, commonly found in the Northeast region. MGR586 can be used for the detection of the variation of rice blast fungal population in Thailand. The results could be employed to assist breeding for rice blast resistance.
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