Indirect immunofluorescence in evaluation of bulls sperm antigenic characteristics
2000
Lazarevic, M. (Fakultet veterinarske medicine, Beograd (Yugoslavia)) | Rodic, S. | Ostojic, J. | Jakovljevic, G. | Jovicin, M.
The investigations were conducted in order to evaluate variations in anti-sperm antibodies in rabbit sera. Rabbits were previously immunized with TRIS - egg yolk extender. Biociphos plus extender (IMV, France) and with content of insemination straws prepared with same extenders. The sperm cells from ejaculates of 12 Black and White Spotted bulls were investigated by indirect immunofluorescence method. Both extenders and content of insemination straws were capable in raising antibody production. Titer of antibodies had the highest values when sperm cells from insemination straws were used for the test.
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