Isolation of blue light regulated genes from phototropism mutants of Arabidopsis thaliana using subtractive hybridization
1999
Janoudi, A.K. | Poff, K.L. (Michigan State University, East Lansing, Michigan (USA). Department of Energy Plant Research Laboratory)
Using subtractive hybridization, we have isolated a cDNA clone representing a blue light-regulated gene in Arabidopsis thaliana. The clone encodes for the auxin-binding glutathione S-transferae Atpm24.1 (GST; EC2.5.1.18). In Arabidopsis thaliana seedlings, transcript levels of this GST are severely diminished in the phototropism null mutant, JK218. In seedlings of the wild-type parent, transcript levels increase with fluence of blue-light irradiation. The fluences of blue light that up-regulate this GST correspond to the fluences that induce first positive phototropism in Arabidopsis. The blue light up-regulation of Atpm24.1 transcription is evident within 20 min. of irradiation. This time corresponds well to the kinetics with which phototropic curvature develops. Atpm24.1 is regulated by blue light but not by red light. Based on these data, a role for this GST in phototropism is suggested.
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