Study on callus induction from anther and inflorescence culture of sorghum [Sorghum bicolor]
1997
Nakamura, S. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Can, N.D. | Yoshida, T.
Five genotypes of Sorghum bicolor Moench and five MS basal media supplemented with to kinds of hormone at various concentration were tested fro their response to anther culture. TX403 produced calli with the highest frequency. Anthers cultured on medium containing 2.0 mgL(-1) kinetin, 3.0 mgL(-1) 2,4-D and 1,500 mgL(-1) gellan gum gave higher callus inducing rate of 1.25% than those cultured on other four medium. Frequency of callus producing decreased as anther aged. Rate of callus forming was high as panicle length ranged from 4.0 to 8.0 cm, corresponding with anther size ranged from 1,200 to 1,750 mu-m. The average number of plantlets regenerated derived from anthers with calli was 14.3%. Chromosome number of 20 from root-tip cells was found for most regenerated plantlets. For inflorescence culture, medium containing 2.5 mgL(-1) kinetin, 3.0 mgL(-1) 2,-4D and 5,000 mgL(-1) agar powder gave callus forming rate of 39.4%. The young inflorescence was an excellent source for inflo
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