Progesterone stimulation by prostaglandin F2alpha involves the protein kinase C pathway in cultured bovine luteal cells
1998
Okuda, K. (Okayama Univ. (Japan). Faculty of Agriculture) | Uenoyama, Y. | Lee, K.W. | Sakumoto, R. | Skarzynski, D.J.
The intracellular mechanism that mediates the stimulatory effect of prostaglandin F(2alpha) (PGF(2alpha)) on progesterone secretion by bovine luteal cells is not well understood. In the present study, we investigated to test the hypothesis that PGF(2alpha) stimulates progesterone secretion through activation of protein kinase C (PKC) by using a cell culture system. Bovine mid-luteal cells (Days 8-12 of the estrous cycle) were cultured for 24 h and then exposed to varying concentrations of PGF(2alpha) (10(-8), 10(-7), 10(-6)M) or luteinizing hormone (LH; 100 ng/ml) for 4, 6 or 16h. Treatment of the cells with the highest dose of PGF(2alpha) (10(-6)M) or LH resulted in increases in progesterone secretion for all three exposure times. Following down-regulation of PKC with a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (PMA; 10(-6)M), the stimulatory effect of PGF(2alpha) was no longer evident, whereas the response to LH was unaffected
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