Embryo transfer of bovine embryos collected from cows with superovulatory treatments following aspiration of a dominant follicle
1998
Kobayashi, S. (Fukui-ken. Government Office (Japan)) | Shozu, S. | Yamada, M.
The objective of this study was to examine pregnancy rate in embryo transfer of embryos produced by IVM/IVF/IVC of oocytes collected from cows superovulated after ultrasound-guided follicular aspiration of a dominant follicle in Japanese Black Cattle. The embryos recovered were frozen according to the two-step freezing method using 10% glycerol as a cryoprotectant (test group). The two frozen embryos or two fresh embryos (control group) were transferred nonsurgically to the uterine horn with the corpus luteum in recipient (Holstein dairy cows). The pregnancy rate did not differ between in test group, 66.7% (13/21) and in control group, 64.9% (24/37), and 4 recipients were delivered of 4 normal calves in test group. Ultrasonographic observations revealed that the number of small follicles dramatically increased on the right and left ovaries by aspiration of a dominant follicle before superovulatory treatments. Superovulatory treatments 2 days after aspiration of a dominant follicle resulted in higher yields of transferable embryos, compared with those in conventional superovulatory treatments. It is concluded that newly formed small follicles recruited by aspiration of a dominant follicle prior to superovulatory treatment could yield much viable and normal embryos
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