Effects of ultraviolet irradiation on genetical inactivation and morphological structure of sperm of the Pacific abalone Haliotis discus hannai
1999
Li, Q. (Tohoku Univ., Sendai (Japan). Faculty of Agriculture) | Osada, M. | Kashihara, M. | Hirohashi, K. | Kijima, A.
Effects of ultraviolet (UV) irradiation on genetical inactivation and morphological structure of sperms were examined in the Pacific abalone. Irradiation for 20 s at a UV intensity of 720/mu-W/square-cm/s was the optimum dose to achieve haploid gynogenesis. The fertilization rate apparently decreased with increasing irradiation time, and the development of the eggs fertilized with the genetically inactivated sperms terminated before reaching the veliger stage. Scanning electron microscopy showed clear destruction of the sperm acrosome and flagellum in the UV-irradiated sperms. As the duration of irradiation increased, the acrosome of sperms tended to suffer greater damage, until the sperms eventually lost their flagella. Abnormalities in these structures have appeared to account, at least in part, for the decline of the fertilization rate of eggs inseminated with UV-irradiated sperms
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