Utilization of Domestic Vegetable Juices as a Medium for Growth and Reproduction of Phytophthora species
1998
Jee Hyeong-Jin | Cho Weon-Dae Division of Plant Pathology, Department of Crop Protection, National Institute of Agricultural Science and Technology, Suwon 441-707 (Korea Republic)
V8 juice agar (V8A) has been the most popular and commonly used medium for growth and reproduction of Phytophthora. However, frequently V8 vegetable juice is not available or difficult to obtain in Koera. We therefore developed widely accessible medium to substitute for V8A using domestically available five juices; two carrot (KCJA), two tomato (KTJA) and a vegetable-mix (KVMA). To prepare 10% juice medium, each vegetable juice 100 ml, DW 900 ml, agar 17g and CaCO3 0.5~1.0 g were supplemented to adjust pH ca. 6.0. Mycelial growth of P. cactorum and P. capsici on KTJA and KVMA was equally effective as V8A in dark, however, KCJA, KTJA and KVMA was slightly better or insignificantly different from V8A for the growth of P. cactorum, P. capsici, P. derchsleri and P. nicotianae under light. Sporangial production of P. cactorum, P. capsici and P. nicotianae on KTJA and KVMA was as good as V8A and slightly better than KCJA, but the difference was insignificant by P. cactorum and P. nicotianae. The four fungi successfully formed oospores on all the media although the numbers were varied among species and media. While KTJA was the best for P. cactorum and P. capsici, V8A was the best for P. capsici and P. drechsleri. However, KCJA stimulated highest number of oospores of P. nicotianae. Overall results showed that domestically available vegetable juices were highly effective on growth and reproduction of Phytophthora and comparable to V8 juice. Therefore, the domestic juice medium can be successfully replaced V8A in Phytophthora study.
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