Analysis and Detection of Coat Protein Gene of Barley Yellow Mosaic Virus and Barley Mild Mosaic Virus by RT-PCR
1998
Lee Kui Jae Dept. of Agricultural Biology, College of Agriculture, Chonbuk Nat'l Univ. Chonju 561-756 (Korea Republic)
Using the reverse transcription polymerase chain reaction (RT-PCR), a rapid and sensitive assay method for the detection and identification of barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) was adapted. Two units of primers from each virus were selected and usde for the determination of two different viruses. PCR fragments of BaYMV (ca. 0.9kb) and BaMMV (ca. 0.8kb) were obtained from the desinged method for the assay of BaYMV and BaMMV coat protein. PT-PCR fragments were cloned using vector pT7 Blue and the sequences of the selected clones were analyzed. Coat protein of BaYMV and that of BaMMV consisted of 297 amino acids (891 nucleotides) and 251 amino acids (753 nucleotides), respectively. The analysis of coat protein genes from these two viruses showed that 45.6% of nucleotides sequence and 34.9% of amino acid in BaYMV were homologous to those in BaMMV.
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