Quantitative analysis of rutin in buckwheat (Fagopyrum sp.) by high performance liquid chromatography [HPLC]
1998
Minami, M. (Shinshu Univ., Minamiminowa, Nagano (Japan). Faculty of Agriculture) | Kitabayashi, H. | Ujihara, A.
For the quantitative analysis of rutin in common buckwheat (Fagopyrum esculentum) and tartary buckwheat (F. tataricum), the operating condition of high performance liquid chromatography (HPLC) and methods of sample preparation and extraction were investigated. Reliable analysis method with less than 5 g of sample was established as follows: 1) drying samples for 24 hours at 70 degrees C by using forced air flow oven, 2) grinding 5 g of seed and 2 g of leaf samples into powder for 30 second, 3) extracting rutin from 1.0 g of common buckwheat powder with 10 min of methyl alcohol and from 0.5 mg of tartary buckwheat seed powder and common buckwheat leaf powder with 20 ml of methyl alcohol at 70 degrees C for 30 min. by using Soxhelt extraction apparatus and water bath, and 4) injecting 20 mu-l of supernatant fluid obtained by centrifugation into chromatograph. The method has high reliability with about 5% of coefficient of variation in rutin content. Establishment of quantitative analysis method of rutin with small amount of seed sample less than 5 g by using HPLC will contribute to the progress of breeding for high rutin content buckwheat varieties
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