Gene introduction into silkworm embryos with a pseudotyped retroviral vector
2000
Komoto, N. (National Inst. of Sericultural and Entomological Science, Tsukuba, Ibaraki (Japan)) | Thibert, C. | Boulo, V. | Fang, Q. | Burns, J.C. | Couble, P.
Gene transfer systems for producing transgenic animals have been investigated using different types of vectors. In this report, we tested pseudotyped retroviral particles for their ability to transfer genes into Bombyx mori. Silkworm eggs were injected with pseudotyped retroviral vectors derived from the Moloney murine leukemia virus carrying the green fluorescent protein coding sequence inserted under the control of a long terminal repeat promoter. PCR analyses and Southern hybridization showed that the vector particles successfully infected Bombyx embryo cells and that the viral genome was reverse transcribed and amplified during development. An inverse PCR assay showed the integration of the vector sequence into the silkworm genome
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