Possibility of using haematological values for rapid diagnosis of iridovirus infection in seabass, Lates calcarifer: Technical paper no.16/1997
1997
Yaowanit Danayadol | Sataporn Direkbusarakom | Lila Ruangpan | Vinai Krachaiwong (Department of Fisheries, Bangkok (Thailand). Songkhla National Inst. of Coastal Aquaculture)
Haematological changes were investigated in each of 30 iridovirus infected and normal seabass (Lates calcarifer). Red blood cells count (RBC), white blood cells count (WBC), hematocrit values (Hct), differential leukocyte count, large sized macrophage cells, swollen macrophage cells and abnormal leukocyte were determined. Histopathological changes and electron microscopic observation were proceed to confirm the sign of iridovirus infection in the samples. Red blood cells count and hematocrit value in the diseased fish were lower than in normal fish but white blood cells count were in the same level as showed by the normal one. Only three kinds leukocyte (lymphocyte, monocyte and neutrophil) were manifested from blood smear of differential leukocyte count. Percentage of neutrophil in infected fish were 4 times higher than in normal fish, while percentage of lymphocyte and monocyte decreased. Large size and swollen macrophage cells from imprinted slide, necrotic white blood cells from blood smear were found in high number in infected fish, while it was very few in normal one. Histopathological changes and electron microscopic studies on the diseased fish samples revealed the parallel results of diagnosis. These data show the possibility of using the large size macrophage cells and necrotic leukocyte for diagnosis the iridovirus infection in seabass. Red blood cells count, hematocrit value and percentage of neutrophil are the minor factors, which can also be the supported values for diagnosis of this disease.
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