Isolation and determination of AXI 1 (Auxin independent) gene's nucleotide sequences from Pisum sativum
1999
Yalcin, M.Y. | Cetiner, S. (Cukurova Univ., Faculty of Agriculture, Adana (Turkey). Div. of Horticulture)
Axi 1 is an auxin independent gene which is characterized only in Nicotiana tabacum and Arabidopsis thaliana. This gene confers on protoplasts the ability to grow in culture not only in the absence of auxin but also at high levels of auxin concentrations. In this study, axi 1 gene was isolated from Pisum sativum and nucleotide sequences of this gene was searched. The nucleotide sequences from Pisum sativum axi 1 gene were compared with the nucleotide sequences of Arabidopsis thaliana axi 1 gene and based on this data, 71.918 % homology between nucleotide sequences and 80.588 % homology between peptide sequences were found. The nucleotide sequences from Pisum sativum axi 1 gene were also compared with the nucleotide sequences of Arabidopsis thaliana axi 1 gene, and as a result, 82.111% homology between nucleotide sequences and also peptide sequences were determined
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