Isolation and preliminary characterization of Streptomyces flavopersicum mutants blocked in streptomycin biosynthesis
2001
Stoilova-Disheva, M. | Peltekova, V. | Lyutzkanova, D. (Institute of Microbiology, Bulgarian Academy of Sciences, Sofia (Bulgaria))
Stable mutants that are blocked in the production of the aminoglycoside antibiotic spectinomycin were generated by treatment of Streptomyces flavopersicus NRRL 2820 with a combination of N-methyl-N-nitro-Nnitrosoguanidine (NTG) and ultraviolet light. On the basis of their co-synthetic properties in agar-strip and mixed culture methods, the mutants were grouped into five phenotypic groups, and arranged in the most probable linear sequence of metabolic blocks.
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