Preparation of diagnostic agents-ingredients for ELISA and use of double sandwich procedures for detection of antigens and antibodies in avian influenza A (grippe)
2000
Hadjiev, G. | Bumbarov, V. (Central Research Veterinary Medical Institute, Sofia (Bulgaria)) | Ivanov, Y. | Kostov, G.
Type-specific antigens from horioallantoic membranes (HAM) and allanto-amnionic fluids (AAF) of chicken embryos (CE), infected with a referent avian influenza virus strain (AIV) subtype H2, as well as corresponding hyperimmune rabbit anf guinea pig sera were prepared. The latter, being highly specific and with a high sensitivity, were used as ingredients in an indirect double-sandwich ELISA procedure for detection of type-specific antigen of AIV and antibodies against it in a blockade double sandwich ELISA procedure. The results of blockade ELISA, applied to 916 hen sera from different farms from different regions of the country and to 11 sera from wild birds revealed no antibodies against AIV. Seven hundried and sixty eight of these sera where parallely studied in agar gel immunodiffusion (AGID) test and the results were negative as well. The studies, performed in the period 1993-1998 for isolation of AIV in Ce from viscera of 212 carcasses from 13 domestic and wild avian species, gave negative results.
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