Unusual behaviour of natural polyphosphates during IMAC [immobilized metal ion affinity chromatography]
2001
Kminkova, M. | Proskova, A. | Kucera, J. (Vyzkumny Ustav Potravinarsky, Prague (Czech Republic))
The atypical course of imino diacetyl agarose saturated with ferric ions of two polyphosphorylated compounds - phytic acid and Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster obtained by the way of pancreatic hydrolysis of bovine casein was detected. The spectral data (for phytic acid) and iron content (for polyphosphate cluster) indicate the strong bond of ferric ions to these polyphosphorylated compounds. The data shows these compounds to bind ferric ion stronger even in comparison with imino diacetate itself. Therefore attention is to be paid to all immobilized metal ion affinity chromatography of natural compounds containing strong chelating groups or several chelating groups in vicinity.
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