Ploidy identification of doubled chromosome number plants in Viola x wittrockiana M 1-generation
2002
Ajalin, I. | Kobza, F. (Mendelova Zemedelska a Lesnicka Univ., Lednice na Morave (Czech Republic). Zahradnicka Fakulta) | Dolezel, J.
The aim of this study was to develop a protocol for production of polyploid M1-generation plants of Viola x wittrockiana. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf blade length/width), and other morphological characteristics of the M1-generation. Secondary screening for novel ploidy levels was performed by flow cytometry (FCM). Hexadecaploid, aneuploid, and mixoploid plants were successfully identified by FCM.
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