The need for understanding mechanisms of resistance: the example of celery | [Le besoin de comprendre les mécanismes de résistance: l'exemple du céleri]
1999
Trumble, J.T. ((University of California, Riverside (Etats Unis). Department of Entomology)) | Diawara, M.M. | Quiros, C.F.
Apium gaveolens L. (var "Conquistador"), the related species A. chilense (A073), A. nodii lorum (A289) and A. prostratum (A230), as well as an F1 hybrid between celery "T.U. 52-75" and A. prostratum and 23 backcross lines obtained by crossing the Fl hybrid back to the celery parent were examined for putative resistance to Liriomyza trifolii. These lines were tested using a no-choice design, so that maximum feeding and oviposition could be expected. Numbers of feeding punctures, mines, pupae, and adults were counted. Of these accessions/lines, several appeared to have good-excellent leafminer resistanceof particular note was 91A-25. Compared to all plants except the wild types, this line had fewer feeding punctures, allowed much less larval development, and no leafminer pupae developed. In order to document if the observed resistance was caused by the carcinogenic and mutagenic linear furanocoumarins, the amounts of psoralen, bergapten and xanthotoxin present in both leaves and petioles of each accession were quantified using high performance liquid chromatography. Line 91A-25, which offered excellent leafminer resistance was found to have nearly 450 microg/g fresh weight of linear furanocoumarins. This is some 25 times the levels known to cause an acute dermatitis in humans. However, for lines with less than 30 g/g of furanocoumarins, regression analyses indicated that substantial resistance was not due to these undesirable compounds. Because of the related nature of the lines tested, our results provided an indication of the inheritance patterns of linear furanocoumarins which could be developed. The implications for use in a breeding program for A. graveolens are discussed
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