Molecular Characterization of Allatostatin cDNA in Central Nervous System and Midgut from the German Cockroach Blattella germanica
2000
Yang Jae-Hyuck | Park Joong-Cheol Korea University, Seoul (Korea Republic)
This study has been carried out to determine molecular characteristics of Blattella germanica allatostatin(AST) inhibiting juvenile hormone(JH) biosynthesis by corpora allata(CA). In order to determine base sequence of coding region in AST gene, a cDNA library was constructed by RT-PCR with total RNA purified from both central nervous system(CNS) and midgut of B. germanica. To get PCR products, PCR and 5'-and 3'-RACE were conducted using degenerated primers designed from the highly conserved region of AST genes from Diploptera punctata and Periplaneta americana. The amplified DNA fragments were subcloned into pGEM-T Easy vector and three cDNA clones obtained were sequenced. They were all composed of 1,506 bp which are able to encode 375 amino acids for synthesis of ASTs in the CNS and midgut of the adult german cockroach. The nucleotide sequence of AST cDNA obtained from B. germanica shows similarities of 83% and 77% to those from D. punctata and P. americana, respectively.
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