Genetic Relationships of Internal Transcribed Spacer (ITS) Regions on Entomopathogenic Fungi by RFLP
Choi In-Young | You Young-Jin Chonbuk-do Agricultural Research and Extension Service, Iksan (Korea Republic)
A similarity coefficient were analyzed by RFLP of fourteen species of entomopathogenic fungi, isolated from inhabiting pupa and adult insect at forest. Each rDNA ITS 1 and ITS 2 with primers of ITS 1 and ITS 4 was amplified by PCR. The amplified products were conserved to 500 bp were not demarcated between genus and species. Four Paeciliomyces tenuipes, two Beauveria bassiana and six Cordyceps militaris were treated by seven restriction enzymes and confirmed in species except JB3 by electrophoresis band. However, the band of C. scarabaeicola showed the identity with B. bassiana. The result of this experiment indicated that the teleomorph of C. scarabaeicola was the same as that of B. bassiana. Cfo1 and Hpa2 of seven restricted enzymes were easily discriminating in the genus between Paecilomyces and Cordyceps. Especially, Cfo1 was more effective to classify the genera of Paecilomyces, Cordyceps and Beauveria than other restriction enzymes.
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