Optimization of Extraction Process for Mass Production of Paclitaxel from Plant Cell Cultures
2000
Kim Jin-Hyun | Hong Seung-Suh Samyang Genex Biotech Research Institute, Taejon (Korea Republic)
Several solvents or combinations of solvents were tested for the extraction of wet or dried biomass at different extraction mode from plant cell cultures. Methanol gave the highest paclitaxel recovery with the least amount of solvent usage. Before extraction, drying of biomass was helpful to decrease solvent usage in extraction step. In this case, drying method was very important to obtain high yield from dried biomass. In the mode of operation, counter-current extraction process can be able to decrease solvent usage, but paclitaxel recovery was almost same with both batch and counter-current mode of operation. The number of extraction times was at least four to obtain high yield (99%) from cell and one to obtain high yield (96%) from cell debris in batch mode. Equilibrium (i.e., the ratio of paclitaxel in biomass to paclitaxel in the extraction solvent) was reached within 5 minutes. The minimum methanol concentration (90%) and solvent amount (biomass:solvent=1 Kg:1L) are enough to obtain high yield (98%) for extraction from biomass.
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