Somatic embryogenesis in coconut (Cocos nucifera L.) var Baybay Tall and Laguna Tall
2001
Patena, L.F. | Laureles, L.R. | Refurzo, L.R.C. | Barba, R.C. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
This study is a component of the biotechnology work on coconut to modify the fatty acid composition of coconut oil to obtain higher laurate content and medium chain triacylglycerols (MCTs) through genetic engineering. In any genetic or transformation work, tissue culture is a vital component. Usually, the bottleneck is a reliable embryogenesis and regeneration protocol since after gene transfer, plants must be regenerated from the transformed tissues to complete the process. Particularly in coconut, no reliable somatic embryogenesis and regeneration protocol has been established to date. For somatic embryogenesis of coconut, 2 varieties (Baybay Tall and Laguna Tall), 4 explants (immature and mature embryos, young inflorescence and plumule), 5 base media (Branton and Blake, Euewens, Murashige and Skoog's MS, Barba and Patena's R and Gamborg's B5) and 3 mango media were tested. Five batches each of mature and immature embryo explants, 3 batches of young inflorescence explants and 4 batches of plumule explants were cultured in vitro. Results showed callus induction using mature and immature embryo and plumule explants in media with 2,4-D. The calli obtained ranged from loose to compact to embryogenic. Primary somatic embryogenesis was observed using immature embryos and plumule explants. This paper presents initial work at IPB on somatic embryogenesis in coconut
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