Isolation, screening and characterization of glucoamylase producing microorganisms
2000
Espino, T.M. | Tambalo, F.Z. | Tambalo, R.D. (Philippines Univ. Los Banos, College, Laguna (Philippines). National Inst. of Molecular Biology and Biotechnology)
Twenty four bacterial isolates and 29 fungal cultures from rotting starchy materials and from Microbial Culture Collection and Service Laboratory (MCCSL) were screened for starch hydrolyzing activities. Ten bacterial isolates and six fungal cultures that showed clear zones of approximately 3 mm were selected for initial production of glucoamylase. The ten bacterial isolates were grown under liquid culture fermentation by using the shake flask method. Bacterial cultures designated as ISO 1, AM 1, AM 2, and AM 3 showed very promising glucoamylase activities using cooked or raw starch as substrates.Bacillus amyloliquefaciens ATCC 23350 was used as the standard organism. Results of the screening by plating the fungal cultures in starch agar medium showed that those organisms obtained from MCCSL had higher starch hydrolyzing activities. The selected fungal cultures were initially tested for glucoamylase production using liquid culture fermentation and solid substrate cultivation. Four fungal cultures namely, Aspergillus niger BIOTECH 3104, Aspergillus awamori NRRL 3112, Aspergillus oryzae NSRI 3860 and Aspergillus oryzae NSRI 3954 showed very promising glucoamylase activities. Morphological and physiological characterization studies of the four selected bacterial isolates indicated that they belong to the Genus Bacillus. Confirmatory tests conducted on the nine isolates using the BBL Crystal GP-ID kit showed that the four bacterial isolates belong to Bacillus subtilis
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