Genetic linkage map of maize for localization of resitance gene against maize downy mildew [Perenossclenospora philippinensis (Weston) Shaw]
2002
Canama, A.O. | Ruswandi, D. | Latiza, M.A. | Balatero, C.H. | Hautea, D.M. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
Breeding for downy mildew resistance is a major breeding objective among corn breeders in the Philippines and other Southeast Asian countries. Advances in molecular marker technologies have enabled the utilization of DNA markers as a tool in marker-aided selection. In order to utilize molecular marker technology to facilitate breeding program for downy mildew resistance, a molecular linkage map was constructed using 142 BC1F1 mapping population derived from a cross between the susceptible parent, Pi 23 and the resistant source P345C4S2B46-2-2-1-2-B-B-B. The linkage map consisted of 52 restriction fragment length polymorphism (RFLP), 33 simple sequence repeats (SSR) and 15 resistance gene analog (RGA) marker loci distributed into 10 linkage groups. The total map length is 1,822 cM with an average distance of 18.22 cM between markers. This represents 90% of the total map length (1,980 cM distributed in 99 bins) published for corn using RFLP-based markers. This linkage map was successfully used to locate quantitative trait loci (QTL) for downy mildew resistance genes
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