Determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate
1998
Nuanchawee Wetprasit | Montri Chulavatnatol (Mahidol Univ., Bangkok (Thailand). Faculty of Science. Dept. of Biochemistry)
Sugar-lectin binding assay was developed as a simple method which employed direct coating of microtiter plate with galactose-binding lectins. Biotin-galactose conjugate was used to bind to the immobilized lectins. The bound conjugate was then detected using streptavidin-horseradish peroxidase. Using the assay in conjuction with various competing carbohydrates, jackfruit lectin from Artocarpus heterophyllus was found to be specific for alpha-anomer of galactose with an aromatic residue.
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