Using microsatellites to study the conservation of black grouse
2000
Segelbacher, G. (Unit of Wildlife Research and Management, Freising (Germany)) | Hoeglund, J.
We outline the use of microsatellite markers to study the conservation biology and genetics of black grouse (Tetrao tetrix) and other grouse species. Microsatellites are useful in conservation genetics because they allow the detection of fine scale genetic structure, migration and possible negative effects of inbreeding in local populations while requiring minute samples of target DNA. Thus non-invasive techniques such as using shed feathers or faecal samples provide enough DNA for PCR-based genotyping. We have developed microsatellite libraries for both black grouse (Tetrao tetrix) and capercaillie (Tetrao urogallus). These markers contain a high number of alleles and allow us to address the questions outlined above. In this paper we show that the markers are variable, and allow genotypic assignment of black grouse to leks separated by only 5.5 km.
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