Post-thaw quality of bull semen frozen in standard and mini doses and it's relation to pregnancy rate in heifers
2002
Padrik, P. | Jaama, Ue. | Kurokin, J. | Jalakas, M. | Aidnik, M. | Majas, L. | Valdmann, A. (Estonian Agricultural University, Tartu (Estonia). Faculty of Veterinary Medicine)
Competitiveness on milk market does not depend only on the amount of investments into technology but also on efficiency of introduction of new genetic material. This is turn depends on availability, price and quality of the best genetic material. There is no major problem to obtain semen from the top dairy bulls but problem is to get good relation between the semen quality and price. One possibility for effective use of bull semen is deep intrauterine insemination of females with minidoses of semen. The aim of our study was to compare the quality parameters of bull spermatozoa after freezing in mini- and standard-doses and establish the parameters what correlate with the pregnancy rate of females after AI. The results of the study showed that sperm motility and membrane hypoosmotic resistance after freezing/thawing were similar in standard and minidoses of semen. Pregnancy rate of heifers after deep intrauterine insemination with minidose was 67.1 per cent while conventional insemination performed twice with standard dose resulted in 61.9 per cent (P0.05) Only Delta HOT-2 estimated in standard-doses correlated significantly with the pregnancy rate of heifers after conventional AI (r=0,98; P0,05). We conclude that freezing of minidoses of semen does not affect sperm quality post-thaw and enables to reach pregnancy rate after deep intrauterine insemination that is equal or even slightly higher than after conventional AI with standard doses of semen
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