Phytochemical and antifungal screening of Medicago sativa and Zinnia elegans
2002
Hafiza, M.A. | Parveen, B. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry) | Ahmad, R. | Hameid, K.
Roots of Medicago sativa (Lucern) and whole plant of Zinnia elegans (Zinnia) were subjected to phytochemical and antifungal screening. M. sativa was found to contain 17% saponins, while Z. elegans had 7.5%, on dry weight basis. Crude saponins isolated from M. sativa showed antifungal activity against Colletotrichum falcatum with minimum inhibitory concentration (MIC) of 289 mug/ml, while Z. elegans inhibited the growth of Fusarium moniliforme up to the concentration of 207 mug/ml. Crude saponins of M. sativa and Z. elegans were fractionated qualitatively into four and five fractions by thin layer chromatography and quantitatively by column chromatography, respectively. Three major fractions of crude saponins isolated from these plants were also checked for their antifungal activity. Fraction A of M. sativa was effective against C. falcatum with MIC of 31 mug/ml, while fraction B of Z. elegans showed pronounced antifungal activity against F. moniliforme having MIC value of 34.5mug/ml.
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