Determination of heat resistance of exponential phase enterococcal cells
2003
Ahmad, M. (University of Azad Jammu and Kashmir (Pakistan). Dept. of Zoology) | Smith, D.G. | Mahboob, S.
The heat resistance of exponential phase cells of environmental barley isolates Enterococcus faecium BAR, and a hospital isolate Enterococcus faecalis M12 grown at 37 or 45 was determined at 55, 60 and 62.5 degree centigrade for 30 min. From the survival curves, the decimal reduction times (D-values) were determined. Cells grown at 45 degree centigrade showed higher D-values. It was determined that the exponential phase cells grown at 45 were more resistant to heat than the heat resistance of cells grown at 37 degree centigrade. The cells of both isolates were found resistant to heat treatment at 55, 60 and 62.5 as compared with the control in which cells were grown at 37 degree centigrade and treated at 62.5 degree centigrade.
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