Effect of growth regulators on the regeneration potential of two sugarcane cultivars SPF-213 and CPF-237
2002
Niaz, F. | Quraishi, A. (National Agricultural Research Centre, Islamabad (Pakistan))
The study was conducted to optimize in vitro plantlets production from callus in two sugarcane cultivars. Sugarcane (Saccharum officinarum cv. SPF-213, CPF- 237) embryogenic calli were induced from young leaves cultured on MS basal medium supplemented with 3.0 mg/l 2,4-D and 0.1 mg/l NAA. Three concentrations of two different growth regulators (6, benzylaminopurine and kinetin) were tested with and without NAA to compare their ability to induce regeneration from embryogenic calli. After 4 weeks of culture, the percentage of shoot induction was evaluated while after 6 weeks, the total number of shoots produced was checked. Medium containing BA at a rate of 1.0 mg performed better than Kinetin with the highest percentage of shoot induction.
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