Effect of cadmium on callus growth in wheat zygotic embrion culture
2003
Kondic-Spika, A. (Naucni institut za ratarstvo i povrtarstvo, Novi Sad (Serbia and Montenegro))
Toxicity effects of cadmium on callus growth and callus tissue dry matter content were investigated. Two winter wheat varieties, Evropa-90 and NSR-5, were used as a material in embryo culture. Isolated embryos were cultivated on a modified MS (Murashige and Skoog, 1962) medium to which Cd was added in three different concentrations (10E-6, 10E-5, and 10E-4 M/l Cd). At the highest Cd concentration (10E-4 M/l), cv. Europa-90 had 90.3 and cv. NSR-5 52.5% of survived calluses. Fresh callus weight was reduced at the concentration of 10E-4 M/l Cd by 51% in cv. Europa-90 and by 57% in cv. NSR-5 in relation to the control. Cadmium decreased the callus dry matter content in cv. NSR-5 only at the highest concentration (10E-4 M/l), while in cv. Evropa-90 Cd had no significant effect on callus dry matter content.
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