Preparation of extracts from needles of omorika (Picea omorika (Pancic) Purkinye) for peroxidase activity and isoenzyme profile analyses
2004
Bogdanovic, J. (University of Belgrade, Belgrade (Serbia and Montenegro). Center for Multidisciplinary Studies) | Milosavic, N. | Vujcic, Z. | Radovic, K.
We studied the effect of nonionic detergent Tween 80 in extraction buffer on the activity and isoenzyme pattern of peroxidase from omorika needles. The activity of soluble peroxidases was studied using UV-VIS spectrophotometry and isoelectrofocusing. Soluble fraction obtained using extraction buffer with detergent had a higher activity and more isoenzymes in IEF pattern in comparison with the corresponding extract not including detergent in extraction medium.
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