Starin differentiation of mycoplasma mycoides subsp. mycoides SC by RAPAD-PCR
2003
Das, Sanjoy | Singh, V.P. | Singh, V.P. (Indian Veterinary Research Institute, Izatnagar (India). Division of Bacteriology and Mycology)
A randomly amplified polymorphic DNA PCR was carried out to differentiate the Indian isolate of M. mycoides subsp. mycoides SC (MmmSC) from the standard strain. Using OPG10 primer, more or less similar RAPD pattern was obtained in both the strains. The bands of 1.48 kbp and 410 bp were present in Indian isolate (NCVP-1/86) but not found in standard PG1 starin, but absent in NCVP-1/86 starin. The prominent bands (500bp, 620bp and 920bp, 1.1kbp and 1.5kbp long intense bands were detected in case of NCVP-1/86 strain.
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