Multiple arbitary amplicon profiling of Trichoderma species known for their potential as biocontrol agents plant pests
2003
Negee, A. | Mukhopadhyaya, P.N. | Kothari, I.L.(Sardar Patel University, VallabhVidhya Nagar (India). Department of Biosciences)
Random polymorphic amplification profiles of two species of the genus Trichoderma (T. reesie and T. harzianum) were generated employing six arbitrarily designed 10-mer oligonucleotide primers. A widely popular genetic distance analysis formula of Nei and Li (1979) was transformed to a computer program [Genetic Distance Analysis (GDA) Program] to aid in rapid analysis of data. Computer simulates of the fingerprint pattern were created for generating composite molecular signature of the organisms. Fifty-seven different alleles could be identified with an average of 4.75 per primer for each species. Primer 2 and 5 with 50% and Primer 3 with 80% GC content generated similar amplicon profiles. On the other hand, Primer 6 with 50% GC content could generate differential fingerprints that indicated a genetic distance of unity. The mean genetic distance computed through analysis of data obtained from all the six primers was found to be 0.28.
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