Synergistic effect of polymines on bulbet multiplication in Lilium sp (asiatic hybrids)
2000
Varshney, Anushri | Dhawan Vibha(Tata Energy Research Institute, New Delhi(India)) | Srivastva, P S(Hamdard University, Hamdard Nagar (India). Center for Biotechnology)
The in vitro bulbet multiplication of Lilium sp. (Asiatic hybrids) enhanced significantly by the application od polyamines than when cultured on medium containing high sucrose and auxin (NAA). Of the two polyamines tested, spermine was more effective. Maximum multiplication Fold was 2.89 for cultivar 'Gran Paradiso' at 150 æM spermine and 2.78 fold for cultivar 'Sanciro' at 50 æM. Putrescine also stimulated bulbet formation and multiplication to the tune of 2.56 and 2.61 fold at 50 æM as compared to control where it was 2.13 and 2.42 fold for cultivars 'Gran Paradiso' and 'Sanciro', respectively. Though polyamines were effective in enhancing multiplication rates on an average of 26% in a year, growth of bulbets remained unaffected.
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