Influence of nutrient deficiency and scurose concentration on the production of artemisinin by cultured cells of sweet wormwood (Artemisia annua L.)
2002
Usha, R. | Swamy, P.M. (Sri Venkateswara University, Tirupati (India). Dept. of Botany)
Sweet wormwood (Artemisia annua L) produces an antimalarial drug artemisinin. Callus cultures of A. annua were subjected to nitrate, phosphate deficiency and sucrose concentrations to study their effects on the callus growth and production of artemisinin content. Nitrate deficiency in the medium caused 1.13 fold increase in the artemisinin. Phosphate and osmotic deficiency resulted in the reduction of artemisinin content. Under conditions of either nitrate deficiency or osmotic stress, growth of callus decreased with a concomitant increase in the artemisinin content. Phosphate deficiency caused increased growth and decreased artemisinin content. However, 2 sucrose concentration in the medium caused maximum increase in the artemisinin content. The results indicated permissive role of carbon, nitrogen and phosphorus in the callus growth and production of artemisinin.
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