Molecular cloning and characterization of S-adenosylmethionine synthetase isolated from suspension culture Cdna Library of oil palm (Elaeis guineensis Jacq.)
2003
Hwang, S.S. (University Putra Malaysia, Selangor (Malaysia). Dept. of Biotechnology) | Cheah, S.C. | Kulaveerasingam, H. | Tan, S.H.
S-adenosylmethionine (AdoMet) syntheses (ATP: L-methionine S- adenosyltransferase EC 2.5.1.6) enzyme catalyzes the only known route for the biosynthesis of AdoMet, by the conversion of L- methionine and ATP. A cDNA clone encoding AdoMet syntheses, designated as OPADO-11A1 was isolated from oil palm suspension culture cDNA library. Southern blot analysis showed that more than one copy of OPADO-11A1 genes might be present in the oil palm genome. We observed that the expression of OPADO-11A1 was induced by wounding and UV irradiation. The mRNA transcript of OPADO-11A1 was highly expressed after 2 h following wounding and no enzyme activity was detected from 9 to 24 hrs after wounding. For UV induction, the mRNA transcripts were detected at the highest levels at 15 to 20 min after induction and expression levels decreased after 20 min. However, no obvious changes of OPADO-11A1 mRNA levels were observed after 2 hrs of ethephon treatment.
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