Changes in glutamine synthetase activity and gene expression during storage of moso bamboo shoot
2004
Matsui, T. | Bhowmik, P.K. | Yokozeki, K. (Kagawa Univ. (Japan). Dept. of Bioresource Production)
Previously isolated cDNA clones encoding glutamine synthetase (GS, EC 6.3.1.2) genes from asparagus and broccoli were observed that the level of transcripts decreased in both plant species during storage. In order to identify factors regulating the postharvest deterioration of another important Asian vegetable bamboo (Phyllostachys edulis) shoot, the present study was conducted to isolate glutamine synthetase (pBA-GS) cDNA clone from a cDNA library prepared from harvested bamboo shoot using reverse transcriptase- polymerase chain reaction. The partial cDNA clone encodes an mRNA of 832 by and the derived amino acid sequence is highly homologous to GS from rice, barely and maize. Northern blot analysis showed decrease level of pBA-GS mRNA until 9 d at 20 degree C, which coincided well with enzyme activity and ammonia accumulation. Results demonstrated that GS activity decrease during the postharvest storage of bamboo shoot which is a highly perishable vegetable like asparagus spears and broccoli head and is likely to be a critical feature of their postharvest physiology.
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