Kinetics of phosphate ions induced invertase synthesis by Saccharomyces cerevisiae
2004
Ikram-ul-Haq | Shafiq, K. | Ali, S. (Government Coll. Univ., Lahore (Pakistan). Botany Dept.)
Invertase (beta-fructofuranosidase) is a high cost enzyme. Aim of present study was to develop an economically feasible synthetic fermentation medium with appropriate supplemented nutrients. Saccharomyces cerevisiae GCB-K5 was used for investigation. Phosphate ions in fermentation medium greatly influence the invertase secretion capacity of yeast cells. By comparing the kinetic parameters, namely product yield coefficient (Yp/x, Yp/s in Amount of enzyme produced mg-1 substrate consumed & mg-1 cell mass produced respectively), growth yield coefficient (Yx/s in mg mg-1) and specific product and growth rate mu (h-1), it was found that when K2HPO4 at the level of 0.020 % was added in medium invertase secretion was increased. Thus it is favorable to use K2HPO4 as a readily available phosphate source for optimal invertase synthesis.
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