Androgenetic response of wheat crosses derived from diverse parental material
2002
Iqbal, M. | Kisana, N.S. | Mujahid, M.Y. | Ahmad, I. | Ahmed, Z. | Asif, M. | Mustafa, S.Z. (National Agricultural Research Centre, Islamabad (Pakistan). Wheat Programme)
An experiment was conducted to study the androgenetic response of twelve wheat (Triticum aestivum L.) crosses derived from diverse parental material including local wheats from high elevation areas of Pakistan. Anthers were cultured on agar solidified Potato-2 and N-6 induction medium. Regeneration was carried out on 190-2 medium. A total of 6920 anthers were cultured and 247 calli/embryoids were obtained from all the crosses. The highest callus induction (15%) was noted for the cross Sannine/Blue Silver/Barbet1 followed by 6.9 for NR 152/ Punjab 96. Of the total 86 regenerated plantlets, 62 were green while 24 were albino. Depending on the genotype, the frequency of callus induction ranged from 0.82 to 15 %, with a mean of 4.84%. Plantlet regeneration ranged from 0 to 100% with an average of 50%. Shagestsi/Blue Silver/Rabe/2* MOSS exhibited 100 % plantlet regeneration for all the calli plated. Total as well as green plantlet regeneration ranged from 0 to 100 %. Green plantlets / 100 anthers varied from 0 to 7 with an average of 1.46 for all the crosses tested. Mean percent callus induction for all the crosses was higher (5.9) on Potato-2 medium than that on N-6 (3.3). Green plantlets/100 anthers were higher on N-6 (2.2) than on P-2 (0.7) medium.
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