Cloning mature cork oak (Quercus suber L.) trees by somatic embryogenesis [Portugal]
2001
Hernandez, I. | Celestino, C. | Martinez, I. | Manjon, J.L. | Diez, J. | Fernandez-Guijarro, B. | Toribio, M.
The development of propagation technologies for clonal production has been proposed in order to improve oak breeding. Somatic embryogenesis is the tissue culture based method more promising to fulfil all the requirements needed for operational genetic improvement of forest trees. Here we report on the induction of somatic embryogenesis in tissues taken from mature cork oak trees. Embryogenic lines were obtained from leaves of 12 out of 19 trees from four locations, by using a basic protocol previously developed for leaves from seedlings. Isolated somatic embryos from some embryogenic lines were germinated and converted into plants, being these steps influenced by the genotype. In vitro synthesis of ectomycorrhizas with Pisolithus tinctorius and Scleroderma verrucosum was achieved. Plants were weaned and transferred into soil
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