Successful blastocys production from native Thai calf oocytes after in vitro fertilization
1996
Mongkol Techakumphu | Wichai Tantasuparak | Jinda Singlor (Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Science. Dept. of Obstetrics Bynaecology and Reproduction)
Oocytes collected from gonadotropin-stimulated calves were matured in vitro for 24 hrs in TCM 199 NaHCO3 + 10 percent FCS supplemented with FSH/LH and Estradiol-17 beta. The matured oocytes (n=66) were fertilized in vitro, with frozen bull semen, after a swimup preparation and were later co-incubated for 18 hrs. The embryos were temporarily transferred into the oviducts of two ewes and one doe for 6-7 days. After tubectomy, the embryos were flushed out. The number of recovered embryos and their stage of development was investigated by stereomicroscopy. The results showed a recovery rate of 53.0 percent (35/66), a cleavage rate of 45.7 percent (16/35) and a blastocyst formation rate of 22.8 percent (8/35). This is the first report of successful blastocyst production from calf oocytes in Thailand.
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