Moderately halophilic and thermophilic bacteria producing neutral protease isolate from hot spring in sourthern Thailand
1998
Somporn Tanskul | Wiparwan Trirattananukul (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Science. Dept. of Microbiology)
Thermophilic bacteria producting neutral protease could be isolated from southern Thailand hot spring. Twenty six strains were isolated from 20 samples from Khao Chaison in Patthalung and 24 strains from 18 samples from Khuan Don in Satun. ST22 strain, from soil in Satun, could produce the highest neutral protease, 3.133 unit/mg protein secreted extracellularly, higher than that of Bacillus stearotheromophilus, 1.574 unit/mg protein. Most characteristics of ST22 corresponded to Bacillus licheniformis. Optimum conditions for the highest yield of protease production were incubation at 50 deg C with shaking (200 rpm) in Frazier Gelatin Medium containing 3 percent (w/v) gelatin, 5 percent (w/v) beef extract and 2 percent (w/v) NaCl, pH 6-7. ST22 could produced the enzyme at late log phase or early stationary phase, about 18 hr after cultivation. Its generation time was 90 min and specific growth rate was 0.462. This is the first report in Thailand.
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