Molecular characterisation of glutenin alleles at the Glu-D1 locus
Vapa, Lj.B.(Faculty of Sciences, Novi Sad (Serbia and Montenegro)) | Obreht, D.R. | Kobiljski, B.Dj. | Davidovic, M.R.
Eighteen bread wheat cultivars developed at Institute of Field and Vegetable Crops in Novi Sad, Serbia (Serbia and Montenegro) were screened for the alleles present at Glu-D1 locus using SDS PAGE and PCR-based approach. The Glu-D1 locus was analysed for two main x-type alleles which code for HMW glutenins 2 and 5, and two main y-type alleles which code for 10 and 12 HMW glutenin subunits. Among the analyzed cultivars, 55.6% expressed the presence of 1Dx5 and 1Dy10 alleles at Glu-D1 locus. They are potentially good bread making quality genotypes, which can be used as parents in wheat breeding programs. Diagnostic PCR system with primers specific for nearly identical alleles represents a useful new tool in market-assisted selection, enabling identification and selection of a single allele, instead of the polypeptide gene product.
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