Rapid axillary bud proliferation and ex vitro rooting of Eupatorium triplinerve
Martin, K.P.(University of Calicut, Kerala (India). Dept. of Biotechnology)E-mail:[email protected]
Effective protocol was established for micropropagation of the medicinal plant Eupatorium triplinerve through rapid axillary bud proliferation and ex vitro rooting. Murashige and Skoog's (MS) medium fortified with 8.87 microM benzylaminopurine (BAP) and 2.46 microM indole-3-butyric acid (IBA) was the best for axillary bud proliferation. It developed 8.1 shoots per node in average. Excision and culture of the node segments of the in vitro shoots on medium supplemented with the same concentration of growth regulators developed more than 30 shoots within 40 d. Shoot multiplication did not exhibit any decrease in the number of shoots even at 7th subculture. Dipping of the basal end of shoots in 2.46 microM IBA solution for 10 d induced roots and its transfer to small pots facilitated the survival of all rooted shoots (100%). Ex vitro rooting by direct transfer of the shoots from multiplication medium showed 92% survival.
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