Production and utilization of doubled haploids in Brassica oleracea vegetables
Klima, M.(Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic))E-mail:[email protected] | Vyvadilova, M. | Kucera, V.
More than 400 regenerants of R1 generation were derived in kohlrabi, cabbage and cauliflower by means of different modifications of microspore culture technique. The highest frequency of embryogenesis and subsequent regeneration of plants were achieved in cauliflower cultivar Siria F1, kohlrabi line P7 and some experimental F1 hybrids of cauliflower. The best production of embryos was obtained when donor plants were grown under controlled light and temperature conditions. The regeneration of plantlets was considerably improved by repeated subculture of cotyledonary embryos on media with various combinations of phytohormones and excision of the cotyledons from mature embryos. The percentage of plant regeneration from subcultured embryos in kohlrabi ranged from 11.11 to 63.64%, in cauliflower from 23.53 to 46.19% and in cabbage from 5.88 to 52.00%.
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