Biosynthesis of folates by lactic acid bacteria and propionibacteria in fermented milk
Holasova, M.(Vyzkumny Ustav Potravinarsky, Prague (Czech Republic))E-mail:[email protected] | Fiedlerova, V.(Vyzkumny Ustav Potravinarsky, Prague (Czech Republic)) | Roubal, P.(Vyzkumny Ustav Mlekarensky, MILCOM, Prague (Czech Republic)) | Pechacova, M.(Vyzkumny Ustav Mlekarensky, MILCOM, Prague (Czech Republic))
The ability of selected strains of Bifidobacterium longum, B. bifidum, Streptococcus thermophilus, and Propionibacterium freudenreichii subsp. shermanii to produce folates was investigated in order to increase folate levels in fermented milk. HPLC was used for the determination of 5-methyltetrahydrofolate (5-MTHF) concentrations. There were marked differences in folate production among the species as well as strains tested. All strains of S. thermophilus tested showed 5-MTHF production. More than six-fold increase in 5-MTHF content was found after 12 h of fermentation in comparison with control. B. longum strains increased the folate content only by maximum 73%, P. freudenreichii subsp. shermanii strains did not influence the 5-MTHF levels. S. thermophilus should be used for natural folate enrichment of fermented dairy products.
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